The Effects of Hepatotoxic Agents and of Liver Growth on the Urinary Excretion of the 7V-Hydroxy Metabolite of 2-Acetylaminofluorene by Rats*

Adult rats subjected to a number of treatments which cause liver damage and/or growth (administration of hepatotoxic agents, partial hepatectomy, or protein deple tion-repletion) and young growing rats excreted in the urine a greater percentage of a test dose of 2-acetylaminofluorene (AAF) as the carcinogenic Af-hydroxy metabolite than did normal adult rats. Alterations in the urinary excretion of the noncarcinogenic ring-hydroxy derivatives of AAF under these conditions were much smaller and, in most cases, negligible. Rats fed certain thermally oxidized derivatives of corn oil excreted 1^-2 times as much W-hydroxy-AAF in the urine after a test dose of AAF as rats fed fresh corn oil. These effects are correlated with the increased carcinogenicity of AAF when fed with the thermally oxidized products (Sugai et al.).